IPO4 Polyclonal antibody

• Featured Product
• KD/KO Validated

IPO4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11679-1-AP

Synonyms

Ran-binding protein 4, Importin-4b, Importin-4, Importin 4b, importin 4

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Validation Data Gallery

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  WB analysis using 11679-1-AP

  HeLa cell lysates were subjected to SDS PAGE followed by western blot with 11679-1-AP (IPO4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11679-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11679-1-AP (IPO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11679-1-AP

  IP result of anti-IPO4 (IP:11679-1-AP, 4ug; Detection:11679-1-AP 1:2000) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human stomach cancer using 11679-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11679-1-AP (IPO4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11679-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11679-1-AP (IPO4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11679-1-AP

  Immunofluorescent analysis of HepG2 cells, using IPO4 antibody 11679-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

11679-1-AP targets IPO4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IPO4 fusion protein Ag2281
• Full Name: importin 4
• Calculated Molecular Weight: 1081 aa, 118 kDa
• Observed Molecular Weight: 118 kDa
• GenBank Accession Number: BC003690
• Gene Symbol: IPO4
• Gene ID (NCBI): 79711
• RRID: AB_2127677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.

Publications

Species	Application	Title
human	WB	Cell Res; Neddylation of PTEN regulates its nuclear import and promotes tumor development.; Authors - Ping Xie
human	WB,IP	Proc Natl Acad Sci U S A; CCAAT/enhancer binding protein delta (C/EBPdelta, CEBPD)-mediated nuclear import of FANCD2 by IPO4 augments cellular response to DNA damage.; Authors - Wang Jun J
human	WB	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB	PLoS One; An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine.; Authors - Echeverría Pablo C PC
human	WB	Biochem Biophys Res Commun; Nuclear import of Nkx2-2 is mediated by multiple pathways.; Authors - Wenbo Lin