IPMK Polyclonal antibody

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  WB analysis using 30161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30161-1-AP (IPMK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30161-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using IPMK antibody (30161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

IPMK Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30161-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

IMPK, IPMK

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30161-1-AP targets IPMK in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IPMK fusion protein Ag32377
• Full Name: inositol polyphosphate multikinase
• Observed Molecular Weight: 42-47 kDa
• GenBank Accession Number: BC065709
• Gene Symbol: IPMK
• Gene ID (NCBI): 253430
• RRID: AB_3086252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NFU5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IPMK plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). It is required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).