INTS7 Polyclonal antibody, PBS Only

INTS7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17054-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 17054-1-AP (same clone as 17054-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17054-1-AP (INTS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 17054-1-AP (same clone as 17054-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 17054-1-AP (INTS7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 17054-1-AP (same clone as 17054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 17054-1-AP (INTS7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 17054-1-AP (same clone as 17054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 17054-1-AP (INTS7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 17054-1-AP (same clone as 17054-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 17054-1-AP (INTS7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

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Product Information

17054-1-PBS targets INTS7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INTS7 fusion protein Ag10755
• Full Name: integrator complex subunit 7
• Calculated Molecular Weight: 948 aa, 105 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC020523
• Gene Symbol: INTS7
• Gene ID (NCBI): 25896
• RRID: AB_2127393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

INTS7 is a subunit of the integrator complex. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase (PMID: 21659603).