Various lysates were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.
WB analysis of HEK-293 using 67060-1-Ig
WB result of INTS3 antibody (67060-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS3 transfected HEK-293 cells.
WB result of INTS3 antibody (67060-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS3 transfected HEK-293 cells.
WB analysis of 4T1 using 67060-1-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
4T1 cells were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF Staining of MCF-7 using 67060-1-Ig
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using INTS3 antibody (67060-1-Ig, Clone: 2G11B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Various lysates were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.
WB analysis of HEK-293 using 67060-1-Ig
WB result of INTS3 antibody (67060-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS3 transfected HEK-293 cells.
WB analysis of 4T1 using 67060-1-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 67060-1-Ig (INTS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF/ICC Figures
IF Staining of MCF-7 using 67060-1-Ig
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using INTS3 antibody (67060-1-Ig, Clone: 2G11B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
INTS3 Monoclonal antibody
Catalog Number
67060-1-Ig
Citations
-
Dilutions
WB : 1:2000-1:10000 IF/ICC : 1:200-1:800
Applications
WB, IF/ICC, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
