human heart tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human heart tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21417-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 21417-1-AP
IP result of anti-INPP5J (IP:21417-1-AP, 4ug; Detection:21417-1-AP 1:500) with mouse lung tissue lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 21417-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 21417-1-AP (INPP5J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 21417-1-AP (INPP5J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
INPP5J(Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A) is also named as PIB5PA, PIPP and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. INPP5J is a key signalling molecule that regulates dendritic outgrowth through activation of small GTPase signalling via interaction between FRMPD4 and ARHGEF7. It can control the resting membrane potential through a specific ion-channel-receptor-ligand interaction that brings about a large conformational change, analogous to neurotransmitter activation of ion channels at synapses. (PMID:22595022). This protein has 3 isoforms produced by alternative splicing.
human heart tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21417-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human kidney using 21417-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 21417-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 21417-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 21417-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse lung using 21417-1-AP
IP result of anti-INPP5J (IP:21417-1-AP, 4ug; Detection:21417-1-AP 1:500) with mouse lung tissue lysate 4000ug.
IF/ICC Figures
IF Staining of HeLa using 21417-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 21417-1-AP (INPP5J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
INPP5J Polyclonal antibody
Catalog Number
21417-1-AP
Citations
3
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
