INPP5E Polyclonal antibody

• Featured Product
• KD/KO Validated

INPP5E Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

49

Reviews (1)

Cat no : 17797-1-AP

Synonyms

Phosphatidylinositol polyphosphate 5-phosphatase type IV, Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, Inositol polyphosphate-5-phosphatase E, EC:3.1.3.86, EC:3.1.3.36

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 17797-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17797-1-AP (INPP5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17797-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17797-1-AP (INPP5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17797-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17797-1-AP (INPP5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17797-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17797-1-AP (INPP5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17797-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17797-1-AP (INPP5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 17797-1-AP

  IP result of anti-INPP5E (IP:17797-1-AP, 3ug; Detection:17797-1-AP 1:1000) with HEK-293 cells lysate 1000ug.

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  IHC staining of human testis using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 17797-1-AP (INPP5E Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 17797-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 17797-1-AP (INPP5E Antibody) at dilution of 1:50.

• 

  IF Staining of hTERT-RPE1 cells using 17797-1-AP

  IF result of ciliary INPP5E in human hTERT-RPE1 cell: Upper is from control siRNA transfected cells and bottom is from INPP5E siRNA transfected cells. 17797-1-AP specifically recognizes the ciliary INPP5E (From Dr.Seongjin Seo).

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  IF Staining of hTERT-RPE1 using 17797-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using INPP5E antibody (17797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  IF Staining of hTERT-RPE1 using 17797-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using INPP5E antibody (17797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, mouse brain tissue, mouse testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human testis tissue, human kidney tissue, human placenta tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 35 publications below
• CoIP: See 1 publications below

Product Information

17797-1-AP targets INPP5E in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INPP5E fusion protein Ag11959
• Full Name: inositol polyphosphate-5-phosphatase, 72 kDa
• Calculated Molecular Weight: 644 aa, 70 kDa
• Observed Molecular Weight: 64-66 kDa
• GenBank Accession Number: BC028032
• Gene Symbol: INPP5E
• Gene ID (NCBI): 56623
• RRID: AB_2167120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.

Publications

Species	Application	Title
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen ; KO Validated
human	IF	Nat Commun; Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.; Authors - Qingwen Xu
		Nat Commun; KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling.; Authors - Kenneth B Schou
mouse, human	WB,IF	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC ; KD Validated
human	IF	Curr Biol; Exocyst complex mediates recycling of internal cilia.; Authors - Félix E Rivera-Molina
mouse	WB	JCI Insight; Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.; Authors - Hairong Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-24-2023)

INPP5E stained in FITC is compared to ARL13b in Cy3. Methanol fixation, Block 1h, incubate 2h with primary ab, and 1h with secondary ab. The antibody is still a bit noisy (despite the blocking at 5% and ab diluted in 2%BSA) but the signal is strong and match what we expect.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/150
• Cell Tissue Type: RPE1

INPP5E Antibody Immunofluorescence validation (1/150 dilution) in RPE1 (Cat no:17797-1-AP)

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