INO80 Recombinant antibody

INO80 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230440E9

Cat no : 83117-4-RR

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259

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Validation Data Gallery

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  IF Staining of HepG2 using 83117-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 83117-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 83117-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ino80 Recombinant Antibody (83117-4-RR, Clone:230440E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83117-4-RR targets INO80 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: INO80 homolog (S. cerevisiae)
• Calculated Molecular Weight: 1556 aa, 177 kDa
• GenBank Accession Number: BC146785
• Gene Symbol: INO80
• Gene ID (NCBI): 54617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.