Inhibin beta A-Specific Polyclonal antibody

Inhibin beta A-Specific Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17524-1-AP

Synonyms

Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, Inhibin β-A, inhibin, beta A

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Validation Data Gallery

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  WB analysis of rat brain using 17524-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 17524-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 17524-1-AP

  IP result of anti-Inhibin beta A-Specific (IP:17524-1-AP, 4ug; Detection:17524-1-AP 1:500) with rat brain tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, PC-3 cells, rat brain tissue
• Positive IP detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17524-1-AP targets Inhibin beta A-Specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inhibin, beta A
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_002192
• Gene Symbol: Inhibin beta A
• Gene ID (NCBI): 3624
• RRID: AB_2296035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibin βA (INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.