ING4-specific Polyclonal antibody

ING4-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16188-1-AP

Synonyms

ING4, Inhibitor of growth protein 4, my036, p29ING4

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Validation Data Gallery

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  WB analysis of Jurkat using 16188-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16188-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 16188-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 16188-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16188-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16188-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16188-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16188-1-AP

  IP result of anti-ING4-specific (IP:16188-1-AP, 3ug; Detection:16188-1-AP 1:300) with HeLa cells lysate 2500ug.

• 

  IHC staining of human brain using 16188-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16188-1-AP (ING4-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16188-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16188-1-AP (ING4-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16188-1-AP (ING4-specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16188-1-AP (ING4-specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16188-1-AP

  Immunofluorescent analysis of HepG2 cells using 16188-1-AP (ING4-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells, mouse colon tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

16188-1-AP targets ING4-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inhibitor of growth family, member 4
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: NM_001127582
• Gene Symbol: ING4
• Gene ID (NCBI): 51147
• RRID: AB_2127798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion. This antibody is specifically against p29ING4.

Publications

Species	Application	Title
human	WB	Oncogene; ING4 regulates a secretory phenotype in primary fibroblasts with dual effects on cell proliferation and tumor growth.; Authors - Moreno A A
human	IHC	PLoS One; Novel multiple markers to distinguish melanoma from dysplastic nevi.; Authors - Zhang Guohong G
		Am J Physiol Heart Circ Physiol; Carvedilol-responsive microRNAs, miR-199a-3p and -214 protect cardiomyocytes from simulated ischemia-reperfusion injury.; Authors - Kyoung-Mi Park
human	WB,IHC,IF	Oral Oncol; Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma.; Authors - Li Xiao-han XH
human	IHC	Acta Histochem; Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1α and snail in HK2 cells.; Authors - Lingling Lu
human	IHC	Mol Med Rep; PMID: 27484725; Authors - Xin Ren