ING4 Monoclonal antibody

ING4 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A12A3

Cat no : 67754-1-Ig

Synonyms

ING4, Inhibitor of growth protein 4, my036, p29ING4

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Validation Data Gallery

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  WB analysis using 67754-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of pig brain using 67754-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit brain using 67754-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67754-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67754-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67754-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67754-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67754-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 67754-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ING4 antibody (67754-1-Ig, Clone: 1A12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67754-1-Ig targets ING4 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, rabbit, rat samples.

• Tested Reactivity: Human, pig, rabbit, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ING4 fusion protein Ag4610
• Full Name: inhibitor of growth family, member 4
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007781
• Gene Symbol: ING4
• Gene ID (NCBI): 51147
• RRID: AB_2918522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion.