ING2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ING2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11560-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11560-1-AP (same clone as 11560-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

  IP result of anti-ING2 (IP:11560-1-AP, 3ug; Detection:11560-1-AP 1:500) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11560-1-AP (same clone as 11560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11560-1-AP (same clone as 11560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 11560-1-AP (same clone as 11560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 11560-1-AP (same clone as 11560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11560-1-AP (ING2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

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Product Information

11560-1-PBS targets ING2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ING2 fusion protein Ag2121
• Full Name: inhibitor of growth family, member 2
• Calculated Molecular Weight: 280 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC030128
• Gene Symbol: ING2
• Gene ID (NCBI): 3622
• RRID: AB_2127771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. It is induced by the DNA-damaging agent neocarzinostatin. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program. Catalog#11560-1-AP is a rabbit polyclonal antibody raised against full length ING2 of human origin.