IMPDH2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IMPDH2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1G8

Cat no : 67663-1-PBS

Synonyms

IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2

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Validation Data Gallery

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  WB analysis using 67663-1-Ig (same clone as 67663-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67663-1-Ig (same clone as 67663-1-PBS)

  WB result of IMPDH2 antibody (67663-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH2 transfected A549 cells. This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67663-1-Ig (same clone as 67663-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67663-1-Ig (same clone as 67663-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 67663-1-Ig (same clone as 67663-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 67663-1-Ig (same clone as 67663-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 67663-1-Ig (same clone as 67663-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67663-1-Ig (same clone as 67663-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using IMPDH2 antibody (67663-1-Ig, Clone: 1B1G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67663-1-PBS targets IMPDH2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IMPDH2 fusion protein Ag17170
• Full Name: IMP (inosine monophosphate) dehydrogenase 2
• Calculated Molecular Weight: 514 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC012840
• Gene Symbol: IMPDH2
• Gene ID (NCBI): 3615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270)