ILK Monoclonal antibody, PBS Only

ILK Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10E4

Cat no : 67724-1-PBS

Synonyms

ILK 2, ILK 1, EC:2.7.11.1, Beta-integrin-linked kinase, 59 kDa serine/threonine-protein kinase

---

Validation Data Gallery

• 

  WB analysis using 67724-1-Ig (same clone as 67724-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood platelets using 67724-1-Ig (same clone as 67724-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67724-1-Ig (same clone as 67724-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 67724-1-Ig (same clone as 67724-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 67724-1-Ig (same clone as 67724-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 67724-1-Ig (same clone as 67724-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 67724-1-Ig (same clone as 67724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67724-1-Ig (ILK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

---

Product Information

67724-1-PBS targets ILK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ILK fusion protein Ag21289
• Full Name: integrin-linked kinase
• Calculated Molecular Weight: 451 aa, 59 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC001554
• Gene Symbol: ILK
• Gene ID (NCBI): 3611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).