CXCR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CXCR2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 20634-1-AP

Synonyms

IL8RB, CXCR 2, CXC R2, C-X-C chemokine receptor type 2, CMKAR2

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Validation Data Gallery

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  WB analysis of L02 using 20634-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20634-1-AP (CXCR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20634-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20634-1-AP (CXCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 20634-1-AP

  IP result of anti-CXCR2 (IP:20634-1-AP, 4ug; Detection:20634-1-AP 1:1000) with HepG2 cells lysate 3200ug.

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  IHC staining of human tonsillitis using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20634-1-AP (CXCR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20634-1-AP (CXCR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20634-1-AP (CXCR2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20634-1-AP (CXCR2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse liver using 20634-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CXCR2 antibody (20634-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20634-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CXCR2 antibody (20634-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: L02 cells, COLO 320 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below

Product Information

20634-1-AP targets CXCR2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCR2 fusion protein Ag14670
• Full Name: interleukin 8 receptor, beta
• Calculated Molecular Weight: 360 aa, 41 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC037961
• Gene Symbol: IL8RB
• Gene ID (NCBI): 3579
• RRID: AB_10693624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL8RB, also named as CXCR2, GRO/MGSA receptor, IL-8 receptor type 2, CDw128b, and CD182, belongs to the G-protein coupled receptor 1 family. It is a receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. It binds to IL-8 with high affinity. IL8RB also binds with high affinity to CXCL3, GRO/MGSA, and NAP-2.

Publications

Species	Application	Title
	WB	Cell Death Dis; Pivotal role of IL-8 derived from the interaction between osteosarcoma and tumor-associated macrophages in osteosarcoma growth and metastasis via the FAK pathway; Authors - Rikito Tatsuno
human	IF	Cell Rep; Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8+ T cell; Authors - Fan Xu
human	WB	Sci Signal; Skewing cPLA2α activity toward oxoeicosanoid production promotes neutrophil N2 polarization, wound healing, and the response to sepsis; Authors - Kenneth D Maus
human	WB	Int J Mol Sci; MyD88 in Macrophages Enhances Liver Fibrosis by Activation of NLRP3 Inflammasome in HSCs.; Authors - Shuang Ge
human	IF	Front Immunol; CXCL5 Downregulation in Villous Tissue Is Correlated With Recurrent Spontaneous Abortion; Authors - Sainan Zhang
human	WB,IHC,IF	Pharmaceuticals (Basel); CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling Pathway; Authors - Hai-Zhou Ji ; KD Validated

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