IL-6 Recombinant antibody, PBS Only (Detector)

IL-6 Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240669A2

Cat no : 83747-6-PBS

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00687-4

  Sandwich ELISA standard curve of MP00687-4, Human IL6 Recombinant Matched Antibody Pair - PBS only. 83747-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0117. 83747-6-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

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  Sample test of MP00687-4

  Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL6.

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Product Information

83747-6-PBS targets IL-6 as part of a matched antibody pair:

MP00687-4: 83747-2-PBS capture and 83747-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 6 (interferon, beta 2)
• Calculated Molecular Weight: 212 aa, 24 kDa
• GenBank Accession Number: BC015511
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.