IL-5 Polyclonal antibody

IL-5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26677-1-AP

Synonyms

EDF, IL 5, IL5, IL-5, Interleukin 5, T cell replacing factor, TRF

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Validation Data Gallery

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  WB analysis of Recombinant protein using 26677-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 26677-1-AP (IL5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26677-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26677-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 26677-1-AP is IL-5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-5 fusion protein Ag24583
• Full Name: interleukin 5 (colony-stimulating factor, eosinophil)
• Calculated Molecular Weight: 134 aa, 15 kDa
• GenBank Accession Number: BC066282
• Gene Symbol: IL5
• Gene ID (NCBI): 3567
• RRID: AB_2880598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Laryngoscope; Eosinophilic Sialodochitis: A Type of Chronic Obstructive Sialadenitis Related to Allergy.; Authors - Wen-Xuan Zhu
human	IHC	Laryngoscope; Primary Hyper-IgE-Related Salivary Gland Disease: A New Disease Entity.; Authors - Wen-Xuan Zhu
human	WB	J Immunol Res; Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p.; Authors - Mei Zheng