IL-4R Polyclonal antibody

IL-4R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28331-1-AP

Synonyms

CD124, IL 4 binding protein, IL 4 receptor subunit alpha, IL 4R alpha, IL 4R subunit alpha, IL 4RA, IL4 BP, IL4R, IL-4R, IL4RA, interleukin 4 receptor, sIL4Ralpha/prot, Soluble IL 4R alpha

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Validation Data Gallery

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  WB analysis using 28331-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 28331-1-AP (IL4R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 28331-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28331-1-AP (IL4R antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28331-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28331-1-AP (IL4R antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Daudi cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28331-1-AP targets IL-4R in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-4R fusion protein Ag28766
• Full Name: interleukin 4 receptor
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: NM_000418
• Gene Symbol: IL-4R
• Gene ID (NCBI): 3566
• RRID: AB_2881115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biomedicines; Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis; Authors - Ying Jiang
human	WB	Cancer Sci; CD44v5 domain regulates crosstalk between TNBC cells and tumor-associated macrophages by enhancing the IL-4R/STAT3 axis; Authors - Yanhua Dai