IL-4 Recombinant antibody, PBS Only (Capture)

IL-4 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240794D7

Cat no : 83894-2-PBS

Synonyms

IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00829-3

  Sandwich ELISA standard curve of MP00829-3, Human IL4 Recombinant Matched Antibody Pair - PBS only. 83894-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0104. 83894-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

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  Sample test of MP00829-3

  Human peripheral blood mononuclear cells (0.5 x 10^6 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 5 μg/mL PHA, and 10 ng/mL recombinant human IL-2. After 7 days of culture, cells were changed into DMEM with 10% fetal bovine serum, 10 ng/mL PMA, 1 μg/mL Ca2+ ionophore, and 5 μg/mL PHA. Cell culture supernate was collected after 1d of stimulation and measured 292.5 pg/mL in stimulated PBMC supernatant, 11.2 pg/mL in unstimulated PBMC supernatant.

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  Sample test of MP00829-3

  Serum of sixteen individual healthy human donors was measured. The human IL4 concentration of detected samples was determined to be 7.97 ng/mL with a range of 2.25 - 19.49 ng/mL.

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  Cytometric bead array standard curve of MP00829-3

  Cytometric bead array standard curve of MP00829-3, IL-4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83894-2-PBS. Detection antibody: 83894-5-PBS. Standard: Eg0104. Range: 0.625-40 ng/mL.

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Product Information

83894-2-PBS targets IL-4 as part of a matched antibody pair:

MP00829-3: 83894-2-PBS capture and 83894-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 4
• Calculated Molecular Weight: 153 aa, 17 kDa
• GenBank Accession Number: BC070123
• Gene Symbol: IL-4
• Gene ID (NCBI): 3565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.