IL3RA/CD123 Recombinant antibody, PBS Only (Capture)

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  WB analysis of HL-60 using 83243-3-RR (same clone as 83243-3-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00176-4

  Sandwich ELISA standard curve of MP00176-4, Human IL3RA Recombinant Matched Antibody Pair - PBS only. 83243-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4452. 83243-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

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  Sample test of MP00176-4

  THP-1 and HL-60 cell lysates were measured. The human ILR3A concentration of detected samples was determined to be 76,268.8 pg/mL (based on a 4.0 mg/mL extract load) in THP-1 cell lysate and 39,279.2 pg/mL (based on a 4.3 mg/mL extract load) in HL-60 cell lysate.

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  IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83243-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82672-1-RR against Human IL3RA/CD123 were performed. The affinity constant is 4.88 nM.

IL3RA/CD123 Uni-rAb^TM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83243-3-PBS

Clone No.240042E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

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Product Information

83243-3-PBS targets IL3RA/CD123 as part of a matched antibody pair:

MP00176-4: 83243-3-PBS capture and 83243-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL3RA/CD123 fusion protein Ag4452
• Full Name: interleukin 3 receptor, alpha (low affinity)
• Calculated Molecular Weight: 378 aa, 43 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC035407
• Gene Symbol: CD123
• Gene ID (NCBI): 3563
• ENSEMBL Gene ID: ENSG00000185291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P26951
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.