IL-3 Polyclonal antibody

IL-3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17835-1-AP

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

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Validation Data Gallery

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  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 17835-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17835-1-AP (IL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17835-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17835-1-AP (IL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Recombinant protein, Recombinant protein protein
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 17835-1-AP is IL-3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-3 fusion protein Ag11839
• Full Name: interleukin 3 (colony-stimulating factor, multiple)
• Calculated Molecular Weight: 152 aa, 17 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC066273
• Gene Symbol: IL-3
• Gene ID (NCBI): 3562
• RRID: AB_10644127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Publications

Species	Application	Title
pig	WB	JTCVS Open; Intramyocardial injection of hypoxia-conditioned extracellular vesicles modulates apoptotic signaling in chronically ischemic myocardium; Authors - Dwight D Harris