IL-2RG Polyclonal antibody

IL-2RG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27377-1-AP

Synonyms

IL2RG, IL-2 receptor subunit gamma, IL 2RG, IL 2R subunit gamma, IL 2 receptor subunit gamma

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Validation Data Gallery

• 

  WB analysis of Jurkat using 27377-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 27377-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27377-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27377-1-AP targets IL-2RG in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-2RG fusion protein Ag26388
• Full Name: interleukin 2 receptor, gamma (severe combined immunodeficiency)
• Calculated Molecular Weight: 369 aa, 42 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC014972
• Gene Symbol: IL2RG
• Gene ID (NCBI): 3561
• RRID: AB_2880857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL-2RA), beta (IL-2RB), and gamma (IL-2RG). IL-2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL-2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.