IL-27RA Polyclonal antibody

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  WB analysis using 12308-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12308-1-AP (IL-27RA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IL-27RA Polyclonal Antibody for WB, ELISA

Cat No. 12308-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

IL27RA, IL 27R alpha, IL 27 receptor subunit alpha, Cytokine receptor-like 1, Cytokine receptor WSX-1

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12308-1-AP targets IL-27RA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-27RA fusion protein Ag2956
• Full Name: interleukin 27 receptor, alpha
• Calculated Molecular Weight: 636 aa, 69 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC028003
• Gene Symbol: IL27RA
• Gene ID (NCBI): 9466
• RRID: AB_3669136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UWB1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-27RA, also known as T-cell cytokine receptor WSX-1 or TCCR, is a unique receptor subunit for the cytokine IL-27. It is expressed in a variety of cells including activated T cells, NK cells, mast cells, endothelial cells, activated B cells, monocytes, Langerhans cells, and activated dendritic cells (PMID: 18378322). The interaction with IL-27 leads to the phosphorylation of Jak and STAT proteins, which are crucial for the regulation of immune responses (PMID: 28536468). IL-27RA plays a significant role in immune cell activation and differentiation, particularly in the context of Th1 and Th17 cells (PMID: 38566992).

Publications

Species	Application	Title
mouse	WB	Life Sci; IL-27 attenuates the TGF-β1-induced proliferation, differentiation and collagen synthesis in lung fibroblasts.; Authors - Zhaoxing Dong