IL-24 Polyclonal antibody

IL-24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12064-1-AP

Synonyms

C49A, FISP, IL 24, IL10B, IL24, IL-24, interleukin 24, mda 7, MDA7, Mob 5, ST16

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Validation Data Gallery

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  WB analysis of Recombinant protein using 12064-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 12064-1-AP (IL-24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human malignant melanoma using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human malignant melanoma using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens)..

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  IHC staining of human lung cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IHC detected in: human malignant melanoma tissue, human prostate cancer tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12064-1-AP targets IL-24 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-24 fusion protein Ag2701
• Full Name: interleukin 24
• Calculated Molecular Weight: 207 aa, 24 kDa
• GenBank Accession Number: BC009681
• Gene Symbol: IL-24
• Gene ID (NCBI): 11009
• RRID: AB_10640304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL24, also named as MDA-7, MDA7 or ST16, belongs to the IL-10 family. It has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. The induction of IL24 by oncogenes may support tumor growth at the early stages of cancer. The observed molecular weight of IL24 is reported between 25 and 35 kDa, a value higher than the deduced molecular weight of 23 kDa. This difference is likely due to post-translational modifications, such as glycosylation of the endogenously produced molecule.

Publications

Species	Application	Title
		Nat Med; Glycemic control in diabetes is restored by therapeutic manipulation of cytokines that regulate beta cell stress.; Authors - Sumaira Z Hasnain
human	WB	Front Oncol; Combination Therapy of Prostate Cancer by Oncolytic Adenovirus Harboring Interleukin 24 and Ionizing Radiation.; Authors - Li-Jun Mao
human	WB	J Exp Clin Cancer Res; Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells.; Authors - Jichen Li
human	WB	MedComm (2020); iPSC-derived NK cells with site-specific integration of CAR19 and IL24 at the multi-copy rDNA locus enhanced antitumor activity and proliferation; Authors - Yuxuan Zhang
human		J Toxicol Environ Health A; Enhancement of keratinocyte survival and migration elicited by interleukin 24 upregulation in dermal microvascular endothelium upon welding-fume exposure; Authors - Maori Kono