IL-22RA1 Polyclonal antibody

IL-22RA1 Polyclonal Antibody for WB, IF, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (2)

Cat no : 13462-1-AP

Synonyms

IL22RA1, IL22R, IL 22RA1, IL 22R alpha 1, IL 22 receptor subunit alpha 1

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Validation Data Gallery

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  WB analysis using 13462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL-22RA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13462-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13462-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IF Staining of human gut using 13462-1-AP

  Staining of IL22RA1 (Cat#13462-1-AP) (2ryAbisA647) on FFPE human gut. The nuclei were counter stained with DAPI (in blue). The IL22RA1 signal is localized in the epithelial compartment which is expected. (Data from Manuel Florez, Laboratory Dre. Naglaa Shoukry, CRCHUM).

• 

  FC experiment of HepG2 using 13462-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human IL-22RA1 (13462-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells, HepG2 cells, HT-29 cells, mouse small intestine tissue, mouse heart tissue
• Positive IF detected in: human gut
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

13462-1-AP targets IL-22RA1 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-22RA1 fusion protein Ag4056
• Full Name: interleukin 22 receptor, alpha 1
• Calculated Molecular Weight: 574 aa, 63 kDa
• Observed Molecular Weight: 63-68 kDa
• GenBank Accession Number: BC029273
• Gene Symbol: IL-22RA1
• Gene ID (NCBI): 58985
• RRID: AB_2248953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-22 (IL-22), a member of the IL-10 family of cytokines, is important for the modulation of tissue responses during inflammation. IL-22 receptor is a heterodimer of the IL-10RB and IL-22RA1. IL-22RA1 belongs to the class II cytokine receptor family. It is expressed in a limited number of tissues including skin, colon, liver, lung, pancreas and kidney. The expression is lack in immune cells such as monocytes, T cells, and NK cells (PMID: 15308104). IL-22RA1 can also form a receptor for IL-20 and IL-24 with IL20RB (PMID: 23793061).

Publications

Species	Application	Title
mouse	FC	PLoS Biol; Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.; Authors - Yu Chen
human	WB	Oxid Med Cell Longev; IL-22 Protects against Biliary Ischemia-Reperfusion Injury after Liver Transplantation via Activating STAT3 and Reducing Apoptosis and Oxidative Stress Levels In Vitro and In Vivo.; Authors - Yi Bai
human	WB	Clin Sci (Lond); HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis.; Authors - Jing Li
human	WB	Apoptosis; Elevated IL-22 in psoriasis plays an anti-apoptotic role in keratinocytes through mediating Bcl-xL/Bax.; Authors - Bo Wang
human	IHC	Biochim Biophys Acta Mol Basis Dis; IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C.; Authors - Yang Che
human	WB	Life Sci; The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.; Authors - Shidi Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (06-13-2022)

Very good antibody for my western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colon cancer cells

FH

Susmita (Verified Customer) (03-11-2022)

Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: PC cell line

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