IL-21 Polyclonal antibody

IL-21 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17625-1-AP

Synonyms

IL 21, IL21, IL-21, interleukin 21, Za11

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Validation Data Gallery

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  IHC staining of mouse skin using 17625-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17625-1-AP (IL-21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 17625-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17625-1-AP (IL-21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

17625-1-AP targets IL-21 in IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-21 fusion protein Ag11838
• Full Name: interleukin 21
• Calculated Molecular Weight: 162 aa, 19 kDa
• GenBank Accession Number: BC066258
• Gene Symbol: IL-21
• Gene ID (NCBI): 59067
• RRID: AB_2878422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin 21 (IL-21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells. IL-21 is preliminarily expressed in activated cluster of differentiation 4 (CD4+) T-cells and natural killer (NK) T-cells. IL-21 receptor (IL-21R) is a class I cytokine receptor, which requires dimerization with the indispensable common gamma chain (γc) subunit to bind IL-21. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. IL-21 mediates apoptosis in B-CLL cells through up-regulation of the BIM (BH3 family member) and enhances both direct and antibody-dependent cellular cytotoxicity in these cells.

Publications

Species	Application	Title
mouse	FC	Mol Cell Neurosci; Statin-modified dendritic cells regulate humoral immunity in experimental autoimmune myasthenia gravis.; Authors - Heng Li
mouse	IHC	Biosci Biotechnol Biochem; Interleukin-21 knockout reduces bone loss in ovariectomized mice by inhibiting osteoclastogenesis; Authors - Junlong Hou
human	IF	Int J Mol Sci; Single-Cell RNA-Sequencing Reveals Peripheral T Helper Cells Promoting the Development of IgG4-Related Disease by Enhancing B Cell Activation and Differentiation; Authors - Zongfei Ji
human	IHC	FASEB J; IL-21 promoting angiogenesis contributes to the development of psoriasis; Authors - Dan Han