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IL-1R1 Recombinant antibody, PBS Only

IL-1R1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240130A3

Cat no : 83420-1-PBS

Synonyms

IL1R1, IL 1R 1, EC:3.2.2.6, D2S1473, CD121A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1R1 Recombinant antibody (83420-1-RR)

    WB analysis using 83420-1-RR (same clone as 83420-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83420-1-RR (IL1R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83420-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83420-1-RR

    Affinity and Kinetic Characterization of 83420-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83420-1-RR against Human IL1R1 were performed. The affinity constant is 8.43 nM.

Product Information

The immunogen of 83420-1-PBS is IL-1R1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL-1R1 fusion protein Ag12602
Full Name interleukin 1 receptor, type I
Calculated Molecular Weight 569 aa, 65 kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberBC075063
Gene Symbol IL1R1
Gene ID (NCBI) 3554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL-1R1 (IL-1 receptor type 1, also known as p80, IL-1R-alpha or CD121a) is a 80 kDa transmembrane glycoprotein and a member of the interleukin-1 receptor family (PMID: 35163653; 29248002). It is a receptor for IL-1α, IL-1β, and interleukin-1 receptor antagonist (IL-1Ra). IL-1α and IL-1β interact with the extracellular domain of IL-1R1, triggering the recruitment of an accessory receptor, the IL-1RAcP, resulting in a functional receptor complex that initiates IL-1R1 signaling cascades (PMID: 35163653). IL-1Ra competes with active IL-1 and blocks binding to their common activating receptor IL-1R1 and, thus, inhibits IL-1 signaling (PMID: 35332327; 11641511). It has been reported that IL-1R1 may also bind IL-38 (PMID: 29248002). IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be released from the cell surface following proteolytic cleavage, generating a soluble form of 55-60 kDa (PMID: 17324958).