IL-1 beta Recombinant antibody, PBS Only (Capture)

IL-1 beta Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240902D6

Cat no : 82696-15-PBS

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

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Validation Data Gallery

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  WB analysis of THP-1 using 82696-15-RR (same clone as 82696-15-PBS)

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-15-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-15-PBS in a different storage buffer formulation.

• 

  WB analysis using 82696-15-RR (same clone as 82696-15-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82696-15-RR (IL1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-15-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00331-2

  Sandwich ELISA standard curve of MP00331-2, Human IL-1 beta Recombinant Matched Antibody Pair - PBS only. 82696-15-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0286. 82696-8-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

• 

  Sample test of MP00331-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA or 10 μg/mL PHA and 10 ng/mL IL-2 for 1 day. The mean IL-1 beta concentration was not determined in unstimulated PBMC supernatant, 5,709.3 pg/mL in PHA stimulated PBMC supernatant and 1,110.8 pg/mL in PHA and IL-2 stimulated PBMC supernatant.

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  Sample test of MP00331-2

  Serum of four individual healthy human donors was measured. The IL-1 beta concentration of detected samples was determined to be 93.4 pg/mL with a range of 20.3 - 131.0 pg/mL.

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  IF Staining of THP-1 using 82696-15-RR (same clone as 82696-15-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated THP-1 cells using IL-1 beta antibody (82696-15-RR, Clone: 240902D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82696-15-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00331-2

  Cytometric bead array standard curve of MP00331-2, IL-1 beta Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82696-15-PBS. Detection antibody: 82696-8-PBS. Standard: Eg0286. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00331-3

  Cytometric bead array standard curve of MP00331-3, IL-1 beta Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82696-15-PBS. Detection antibody: 82696-12-PBS. Standard: Eg0286. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 82696-15-RR

  Biolayer interferometry (BLl) kinetic assays of 82696-15-RR against Human IL-1 beta were performed. The affinity constant is 2.09 nM.

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Product Information

82696-15-PBS targets IL-1 beta as part of a matched antibody pair:

MP00331-2: 82696-15-PBS capture and 82696-8-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00331-3: 82696-15-PBS capture and 82696-12-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 269 aa, 31 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC008678
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.