IL-1 beta Polyclonal antibody

• 

  WB analysis using 30234-1-AP

  Untreated, LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 30234-1-AP (IL1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 30234-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 30234-1-AP (IL1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IL-1 beta Polyclonal Antibody for WB, ELISA

Cat No. 30234-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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Tested Applications

• Positive WB detected in: THP-1 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

30234-1-AP targets IL-1 beta in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-1 beta fusion protein Ag30574
• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_000576
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• ENSEMBL Gene ID: ENSG00000125538
• RRID: AB_3086272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01584
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets

Publications

Species	Application	Title
mouse,rat	IF	Turk J Med Sci; Vitamin D3 alleviates intestinal injury in necrotizing enterocolitis and lipopolysaccharide-induced inflammatory response in dendritic cells in rats; Authors - Bo Ke