IL-1 alpha Polyclonal antibody

IL-1 alpha Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 16765-1-AP

Synonyms

IL1A, IL 1 alpha, Hematopoietin-1, IL-1,IL1, IL1 ALPHA

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Validation Data Gallery

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  WB analysis using 16765-1-AP

  Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL-1 alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16765-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16765-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16765-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 16765-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16765-1-AP (IL-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 16765-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16765-1-AP (IL-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 16765-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 16765-1-AP (IL 1 alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PMA, LPS and Brefeldin A treated THP-1 cells, human placenta tissue, mouse liver tissue, mouse lung tissue
• Positive IHC detected in: human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 22 publications below
• IHC: See 13 publications below
• IF: See 10 publications below
• ELISA: See 1 publications below

Product Information

16765-1-AP targets IL-1 alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-1 alpha fusion protein Ag10339
• Full Name: interleukin 1, alpha
• Calculated Molecular Weight: 271 aa, 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC013142
• Gene Symbol: IL-1 alpha
• Gene ID (NCBI): 3552
• RRID: AB_10641044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.

Publications

Species	Application	Title
mouse	IHC	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	IF	Nat Commun; Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment; Authors - Meilin Xue
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang
		Cancer Lett; Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer; Authors - Prabhakar Pitta Venkata
rat	WB	Bioact Mater; Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.; Authors - Liwen Luo
human	IF	Cell Death Discov; Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer.; Authors - Lulin Zhou