IL-18R1/CD218a Recombinant monoclonal antibody

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  WB analysis of NK-92 using 84390-5-RR

  NK-92 cells were subjected to SDS PAGE followed by western blot with 84390-5-RR (IL18R1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NK-92 using 84390-5-RR

  Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using IL-18R1/CD218a antibody (84390-5-RR, Clone: 241673G6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84390-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84390-5-RR against Human IL-18R1/CD218a were performed. The affinity constant is 0.948 nM.

IL-18R1/CD218a Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84390-5-RR

Clone No.241673G6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IL18R1, IL 18R1, IL 18R 1, EC:3.2.2.6, CD218a

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Tested Applications

• Positive WB detected in: NK-92 cells
• Positive IF/ICC detected in: NK-92 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84390-5-RR targets IL-18R1/CD218a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: interleukin 18 receptor 1
• Calculated Molecular Weight: 541 aa, 62 kDa
• Observed Molecular Weight: 60-75 kDa
• GenBank Accession Number: BC093975
• Gene Symbol: IL18R1
• Gene ID (NCBI): 8809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13478
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-18R1 (CD218a) is a component of the interleukin-18 receptor (IL-18R) complex, which is critical for the signaling of IL-18, a pro-inflammatory cytokine. This receptor complex, consisting of IL-18R1 and the IL-18 receptor accessory protein (IL-18RAP), belongs to the immunoglobulin-like family of interleukin-1 receptors (PMID: 35461924). IL-18R1 plays a significant role in inducing the production of interferon-gamma (IFN-γ) and other inflammatory cytokines, contributing to innate and adaptive immune responses (PMID: 2550053). It is also suggested to play a role in hypertension and chronic kidney disease, where the IL-18/IL-18R1 signaling axis may have diagnostic and therapeutic potential (PMID: 35461924).