IL-17A Polyclonal antibody
IL-17A Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, ELISA, Cell treatment
Conjugate
Unconjugated
Cat no : 13082-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, human stomach cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 12 publications below |
IHC | See 25 publications below |
IF | See 11 publications below |
FC | See 1 publications below |
Product Information
13082-1-AP targets IL-17A in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IL-17A fusion protein Ag3733 |
Full Name | interleukin 17A |
Calculated Molecular Weight | 155 aa, 18 kDa |
GenBank Accession Number | BC067505 |
Gene Symbol | IL-17A |
Gene ID (NCBI) | 3605 |
RRID | AB_10644322 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased. This antibody got 32-35 kDa band in western blot, maybe due to homodimer formation and differential glycosylations.
Protocols
Product Specific Protocols | |
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IHC protocol for IL-17A antibody 13082-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Biol Macromol Chitosan-modified dihydromyricetin liposomes promote the repair of liver injury in mice suffering from diabetes mellitus | ||
J Autoimmun IL-17 is expressed on beta and alpha cells of donors with type 1 and type 2 diabetes. | ||
J Med Chem Discovery of Orally Available Retinoic Acid Receptor-Related Orphan Receptor γ-t/Dihydroorotate Dehydrogenase Dual Inhibitors for the Treatment of Refractory Inflammatory Bowel Disease. | ||
J Invest Dermatol Reduced Il17a expression distinguishes a Ly6c(lo)MHCII(hi) macrophage population promoting wound healing. | ||
Arch Oral Biol Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium. | ||
Cell Physiol Biochem Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis. |