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IL-12RB1 Polyclonal antibody

IL-12RB1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13287-1-AP

Synonyms

CD212, IL 12 receptor beta component, IL 12 receptor subunit beta 1, IL 12R beta 1, IL 12R BETA1, IL 12R subunit beta 1, IL 12RB1, IL-12 R beta 1, IL12R, IL12RB, IL12RB1, IL-12RB1



Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13287-1-AP targets IL-12RB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-12RB1 fusion protein Ag3983
Full Name interleukin 12 receptor, beta 1
Calculated Molecular Weight 662 aa, 73 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC029121
Gene Symbol IL12RB1
Gene ID (NCBI) 3594
RRIDAB_2124039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Protocols

Product Specific Protocols
WB protocol for IL-12RB1 antibody 13287-1-APDownload protocol
IHC protocol for IL-12RB1 antibody 13287-1-APDownload protocol
IP protocol for IL-12RB1 antibody 13287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, monkeyWB, IP, IF

J Immunol

Inflammatory signals direct expression of human IL12RB1 into multiple distinct isoforms.

Authors - Ford Nicole R NR
mouseIHC,IF

Front Med (Lausanne)

Serum IL-12p40: A novel biomarker for early prediction of minimal change disease relapse following glucocorticoids therapy

Authors - Mengqiu Bai