IL-12 p70 Polyclonal antibody

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  WB analysis using 30756-1-AP

  Recombinant protein Eg0742 (IL-12 p70) was subjected to SDS PAGE followed by western blot with 30756-1-AP (IL12(IL12B+IL12A) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30756-1-AP

  Recombinant protein Eg0299 (IL-12 P40) was subjected to SDS PAGE followed by western blot with 30756-1-AP (IL12(IL12B+IL12A) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IL-12 p70 Polyclonal Antibody for WB, ELISA

Cat No. 30756-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

IL12, IL-12, IL-12 p70

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30756-1-AP targets IL-12 p70 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: IL-12 p70
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• RRID: AB_3086410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43432(IL12B);P43431(IL12A)
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.