IL-11RA Polyclonal antibody

IL-11RA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10264-1-AP

Synonyms

IL11RA, IL-11R subunit alpha, IL-11 receptor subunit alpha, IL 11RA, IL 11R subunit alpha



Tested Applications

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman prostate cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inN2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10264-1-AP targets IL-11RA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-11RA fusion protein Ag0373
Full Name interleukin 11 receptor, alpha
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC003110
Gene Symbol IL11RA
Gene ID (NCBI) 3590
RRIDAB_2125660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-11 (IL-11), a member of the IL-6 family of cytokines, was firstly identified in bone marrow-derived stromal cells (PMID: 2145578). IL-11 is a multifunctional cytokine that plays important roles in hematopoiesis, bone development, tissue repair, and tumor development (PMID: 33863879). IL-10 signals through a receptor complex consisting of IL-11 receptor alpha subunit (IL-11RA) and gp130. IL-11RA binds IL-11 and gp130 transmits signals to the nucleus via Janus kinase (JAK) activation. In addition to a membrane-bound form, IL-11RA can also exist as a soluble form (sIL-11RA) that acts as an agonist of IL-11 activity (PMID: 26876177; 27493449).

Protocols

Product Specific Protocols
WB protocol for IL-11RA antibody 10264-1-APDownload protocol
IHC protocol for IL-11RA antibody 10264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

EMBO J

Identification of a gene regulatory network associated with prion replication.

Authors - Masue M Marbiah
mouseWB

ACS Nano

Nanoparticle-Mediated RNA Therapy Attenuates Nonalcoholic Steatohepatitis and Related Fibrosis by Targeting Activated Hepatic Stellate Cells

Authors - Chenshuang Zhang