IL-11RA Polyclonal antibody

IL-11RA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10264-1-AP

Synonyms

IL11RA, IL-11R subunit alpha, IL-11 receptor subunit alpha, IL 11RA, IL 11R subunit alpha

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Validation Data Gallery

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  WB analysis using 10264-1-AP

  Mouse colon tissue was subjected to SDS PAGE followed by western blot with 10264-1-AP (IL-11RA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10264-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10264-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10264-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10264-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of N2a cells using 10264-1-AP

  IF result of anti-IL11RA (10264-1-AP, 1:200) with N2a cells (4%PFA fixed) by Miss Masue Marbiah, PhD student MRC Prion Unit, Department of Neurodegenerative Diseases, UCL Institute of Neurology.

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Tested Applications

• Positive WB detected in: mouse colon tissue
• Positive IHC detected in: human prostate cancer tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: N2a cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

10264-1-AP targets IL-11RA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-11RA fusion protein Ag0373
• Full Name: interleukin 11 receptor, alpha
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC003110
• Gene Symbol: IL11RA
• Gene ID (NCBI): 3590
• RRID: AB_2125660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-11 (IL-11), a member of the IL-6 family of cytokines, was firstly identified in bone marrow-derived stromal cells (PMID: 2145578). IL-11 is a multifunctional cytokine that plays important roles in hematopoiesis, bone development, tissue repair, and tumor development (PMID: 33863879). IL-10 signals through a receptor complex consisting of IL-11 receptor alpha subunit (IL-11RA) and gp130. IL-11RA binds IL-11 and gp130 transmits signals to the nucleus via Janus kinase (JAK) activation. In addition to a membrane-bound form, IL-11RA can also exist as a soluble form (sIL-11RA) that acts as an agonist of IL-11 activity (PMID: 26876177; 27493449).

Publications

Species	Application	Title
mouse	IF	EMBO J; Identification of a gene regulatory network associated with prion replication.; Authors - Masue M Marbiah
mouse	WB	ACS Nano; Nanoparticle-Mediated RNA Therapy Attenuates Nonalcoholic Steatohepatitis and Related Fibrosis by Targeting Activated Hepatic Stellate Cells; Authors - Chenshuang Zhang