IL-7 Recombinant antibody

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  WB analysis using 83672-1-RR

  Mouse IL-7 Recombinant proteins(Eg0403) and human IL-7 Recombinant protein (Eg0830 ) were subjected to SDS PAGE followed by western blot with 83672-1-RR (IL-7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83672-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83672-1-RR against Mouse IL-7 were performed. The affinity constant is 3.70 nM.

IL-7 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83672-1-RR

Clone No.240343C8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Il7, interleukin-7, interleukin 7, Il 7, 240343C8

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83672-1-RR targets IL-7 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 7
• Calculated Molecular Weight: 18kd
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_008371.5
• Gene Symbol: Il7
• Gene ID (NCBI): 16196
• RRID: AB_3671277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q544C8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)