IL-6 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IL-6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3B4

Cat no : 66146-1-PBS

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

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Validation Data Gallery

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  WB analysis using 66146-1-Ig (same clone as 66146-1-PBS)

  Non-treated and protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66146-1-Ig (same clone as 66146-1-PBS)

  Non-treated, LPS and protein transport inhibitor treated HUVEC cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66146-1-Ig (same clone as 66146-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

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  WB analysis of Recombinant protein using 66146-1-Ig (same clone as 66146-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 66146-1-Ig (same clone as 66146-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed LPS and Brefeldin A treated HUVEC cells using IL-6 antibody (66146-1-Ig, Clone: 1A3B4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66146-1-PBS in a different storage buffer formulation.

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Product Information

66146-1-PBS targets IL-6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-6 fusion protein Ag10646
• Full Name: interleukin 6 (interferon, beta 2)
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC015511
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.