IL-36 Beta/IL-1F8 Monoclonal antibody, PBS Only

IL-36 Beta/IL-1F8 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

10D12B1

Cat no : 60290-1-PBS

Synonyms

FIL1, FIL1 (ETA), FIL1 eta, FIL1H, IL 1 eta, IL 1F8, IL 1H2, IL1 ETA, IL1F8, IL-1F8, IL1H2, IL-36 beta, IL-36 Beta/IL-1F8, IL36B, Interleukin 1 eta, Interleukin 1 family member 8, Interleukin 1 homolog 2, Interleukin-36 beta

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Validation Data Gallery

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  WB analysis of Recombinant protein using 60290-1-Ig (same clone as 60290-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60290-1-Ig (IL1F8 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60290-1-Ig (same clone as 60290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60290-1-Ig (IL36 beta/IL1F8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60290-1-Ig (same clone as 60290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60290-1-Ig (IL36 beta/IL1F8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60290-1-Ig (same clone as 60290-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-36 Beta/IL-1F8 antibody (60290-1-Ig, Clone: 10D12B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60290-1-Ig (same clone as 60290-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-36 Beta/IL-1F8 antibody (60290-1-Ig, Clone: 10D12B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

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Product Information

60290-1-PBS targets IL-36 Beta/IL-1F8 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-36 Beta/IL-1F8 fusion protein Ag12675
• Full Name: interleukin 1 family, member 8 (eta)
• Calculated Molecular Weight: 157 aa, 18 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC101833
• Gene Symbol: IL-36 Beta/IL-1F8
• Gene ID (NCBI): 27177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.