IL-36 Gamma Polyclonal antibody

• Featured Product
• KD/KO Validated

IL-36 Gamma Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24723-1-AP

Synonyms

IL 1 epsilon, IL 1 related protein 2, IL 1F9, IL 1H1, IL 1RP2, IL1E, IL1F9, IL-1F9, IL1H1, IL1RP2, IL-36 gamma, IL-36γ, Interleukin 1 epsilon, Interleukin 1 family member 9, interleukin 1 family, member 9, Interleukin 1 homolog 1

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Validation Data Gallery

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  IHC staining of human colon cancer using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, human pancreas tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

24723-1-AP targets IL-36 Gamma in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-36 Gamma fusion protein Ag21489
• Full Name: interleukin 1 family, member 9
• Calculated Molecular Weight: 169 aa, 19 kDa
• GenBank Accession Number: BC098337
• Gene Symbol: IL-36 gamma/IL-1F9
• Gene ID (NCBI): 56300
• RRID: AB_2879690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-36 Gamma is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection.

Publications

Species	Application	Title
human	IHC	J Cutan Pathol; IL-36α and IL-36γ expressions in the differential diagnosis of palmoplantar psoriasis and palmoplantar eczema: A retrospective histopathologic and immunohistochemical study.; Authors - Ozan Erdem
human	WB	Sci Immunol; Myeloid cell-derived proteases produce a proinflammatory form of IL-37 that signals via IL-36 receptor engagement; Authors - Graeme P Sullivan ; KD Validated