IL-31 Polyclonal antibody, PBS Only

IL-31 Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22859-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of human tonsillitis using 22859-1-AP (same clone as 22859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22859-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22859-1-AP (same clone as 22859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22859-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22859-1-AP (same clone as 22859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22859-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22859-1-AP (same clone as 22859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22859-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 22859-1-AP (same clone as 22859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22859-1-AP (IL31 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22859-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 22859-1-AP (same clone as 22859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22859-1-AP (IL31 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22859-1-PBS in a different storage buffer formulation.

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Product Information

22859-1-PBS targets IL-31 in IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-31 fusion protein Ag18848
• Full Name: interleukin 31
• Calculated Molecular Weight: 164 aa, 18 kDa
• GenBank Accession Number: BC132998
• Gene Symbol: IL-31
• Gene ID (NCBI): 386653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IL-31 mRNA encodes a 164-aa precursor and a predicted 141-aa mature polypeptide, displaying an apparent molecular mass of 24 kDa in its glycosylated form. IL31 is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.