IL-3 Recombinant antibody, PBS Only (Capture/Detector)

IL-3 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240417G4

Cat no : 83544-4-PBS

Synonyms

IL3, Multipotential colony-stimulating factor, Interleukin-3, Interleukin 3, IL 3

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP00526-4

  Sandwich ELISA standard curve of MP00526-1, IL-3 Recombinant Matched Antibody Pair - PBS only. 83544-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0829.83544-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

• 

  Sample test of MP00526-4

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean IL-3 concentration was determined to be 43.17 pg/mL in unstimulated PBMC supernatant, 676.99 pg/mL in PHA stimulated PBMC supernatant.

• 

  Sample test of MP00526-4

  Serum of seven individual healthy human donors was measured. The IL-3 concentration of detected samples was determined to be 20.87 pg/mL with a range of 5.19 - 65.92 pg/mL.

• 

  Cytometric bead array standard curve of MP00526-1

  Cytometric bead array standard curve of MP00526-1, IL-3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83544-1-PBS. Detection antibody: 83544-4-PBS. Standard: Eg0829. Range: 0.313-40 ng/mL.

• 

  Cytometric bead array standard curve of MP00526-3

  Cytometric bead array standard curve of MP00526-3, IL-3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83544-4-PBS. Detection antibody: 83544-2-PBS. Standard: Eg0829. Range: 0.313-20 ng/mL.

---

Product Information

83544-4-PBS targets IL-3 as part of a matched antibody pair:

MP00526-1: 83544-1-PBS capture and 83544-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00526-3: 83544-4-PBS capture and 83544-2-PBS detection (validated in Cytometric bead array)

MP00526-4: 83544-4-PBS capture and 83544-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 3 (colony-stimulating factor, multiple)
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: NM_000588.3
• Gene Symbol: IL-3
• Gene ID (NCBI): 3562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.