IL-22RA2 Monoclonal antibody, PBS Only

IL-22RA2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5G11A9

Cat no : 66190-1-PBS

Synonyms

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16

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Validation Data Gallery

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  WB analysis of Raji using 66190-1-Ig (same clone as 66190-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66190-1-Ig (same clone as 66190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 66190-1-Ig (same clone as 66190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66190-1-Ig (same clone as 66190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66190-1-Ig (same clone as 66190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66190-1-Ig (same clone as 66190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66190-1-Ig (same clone as 66190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66190-1-Ig (same clone as 66190-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-22RA2 antibody (66190-1-Ig, Clone: 5G11A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66190-1-Ig (same clone as 66190-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-22RA2 antibody (66190-1-Ig, Clone: 5G11A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

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Product Information

66190-1-PBS targets IL-22RA2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-22RA2 fusion protein Ag18682
• Full Name: interleukin 22 receptor, alpha 2
• Calculated Molecular Weight: 263 aa, 31 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC125167
• Gene Symbol: IL-22RA2
• Gene ID (NCBI): 116379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-22 receptor subunit alpha-2 (IL22RA2) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Alternative splicing results in multiple transcript variants encoding distinct isoforms.