IL-21R Polyclonal antibody, PBS Only

• 

  WB analysis using 10533-1-AP (same clone as 10533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 10533-1-AP (same clone as 10533-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 10533-1-AP (same clone as 10533-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 10533-1-AP (same clone as 10533-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10533-1-AP (same clone as 10533-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 10533-1-AP (same clone as 10533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 10533-1-AP (same clone as 10533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

• 

  IF Staining of COLO 320 using 10533-1-AP (same clone as 10533-1-PBS)

  Immunofluorescent analysis of COLO 320 cells using 10533-1-AP (IL21R antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10533-1-PBS in a different storage buffer formulation.

IL-21R Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 10533-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

---

Product Information

10533-1-PBS targets IL-21R in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-21R fusion protein Ag0762
• Full Name: interleukin 21 receptor
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC004348
• Gene Symbol: IL-21R
• Gene ID (NCBI): 50615
• RRID: AB_2124107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HBE5
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.