Anti-Mouse IL-2 Rabbit Recombinant Antibody, PBS Only

IL-2 Uni-rAb^TM Recombinant Antibody for FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240358A2

Cat no : 98055-1-PBS

Synonyms

Il2, Interleukin-2, interleukin 2, Il 2, 240358A2

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP00533-3

  Sandwich ELISA standard curve of MP00533-3, mouse IL-2 Recombinant Matched Antibody Pair - PBS only. 98055-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0762. 83547-1-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

• 

  Sample test of MP00533-3

  EL-4 mouse lymphoblast cells (1 x 10^6  cells/mL) were cultured for 3 days in DMEM supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL PHA and 10 ng/mL PMA. The mean mouse IL-2 concentration was determined to be 16.1 pg/mL in unstimulated EL-4, 309.9 ng/mL in PHA and PMA stimulated EL-4 supernatant.

• 

  Sample test of MP00533-3

  Serum of sixteen mice was measured. were measured. The mouse IL-2 concentration was determined to be 2.27 pg/mL with a range of 1.1-79.9 pg/mL.

• 

  FC experiment of C57BL/6 mouse splenocytes using 98055-1-RR (same clone as 98055-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 mouse splenocytes were intracellularly stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug Anti-Mouse IL-2 Rabbit Recombinant Antibody (98055-1-RR, Clone:240358A2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98055-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98055-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98055-1-RR against Mouse IL-2 were performed. The affinity constant is 0.229 nM.

---

Product Information

98055-1-PBS targets IL-2 as part of a matched antibody pair:

MP00533-3: 98055-1-PBS capture and 83547-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 2
• Calculated Molecular Weight: 19 kDa
• GenBank Accession Number: NM_008366
• Gene Symbol: IL-2
• Gene ID (NCBI): 16183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.