IL-2 Recombinant antibody

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  WB analysis using 83558-5-RR

  Untreated, PMA, A23187 and Brefeldin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83558-5-RR (IL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83558-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83558-5-RR against Human IL-2 were performed. The affinity constant is below 1 pM.

IL-2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83558-5-RR

Clone No.240416H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IL2, TCGF, Interleukin-2, interleukin 2, IL 2

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83558-5-RR targets IL-2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 2
• Calculated Molecular Weight: 153 aa, 18 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC070338
• Gene Symbol: IL2
• Gene ID (NCBI): 3558
• RRID: AB_3671174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P60568
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.