IL-2 Monoclonal antibody, PBS Only

IL-2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11C5

Cat no : 60306-1-PBS

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

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Validation Data Gallery

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  WB analysis using 60306-1-Ig (same clone as 60306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60306-1-Ig (same clone as 60306-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60306-1-PBS targets IL-2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-2 fusion protein Ag10199
• Full Name: interleukin 2
• Calculated Molecular Weight: 153 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC070338
• Gene Symbol: IL2
• Gene ID (NCBI): 3558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.