IL-1RA Polyclonal antibody

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  WB analysis using 31127-1-AP

  Mouse skin tissues were subjected to SDS PAGE followed by western blot with 31127-1-AP (IL-1RN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31127-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IL-1RN antibody (31127-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IL-1RA  Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31127-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra

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Tested Applications

• Positive WB detected in: mouse skin tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31127-1-AP targets IL-1RA  in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 1 receptor antagonist
• Calculated Molecular Weight: 18KD
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: NM_031167.5
• Gene Symbol: IL-1RA
• Gene ID (NCBI): 16181
• RRID: AB_3669864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q542W1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The interleukin-1 receptor antagonist (IL-1RN) belongs to the IL-1 family. L1RN is a protein that binds to IL1 receptors, and inhibits the binding of IL1-alpha and IL1-beta.