IL-1R1 Recombinant antibody

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  WB analysis of mouse liver using 84094-6-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 84094-6-RR (IL-1R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of EL-4-B5 using 84094-6-RR

  EL-4-B5 cells were subjected to SDS PAGE followed by western blot with 84094-6-RR (IL-1R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84094-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84094-6-RR (IL-1R1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84094-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84094-6-RR (IL-1R1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 84094-6-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using IL-1R1 antibody (84094-6-RR, Clone: 241392G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84094-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84094-6-RR against Mouse IL-1R1 were performed. The affinity constant is below 1 pM.

IL-1R1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 84094-6-RR

Clone No.241392G7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IL-1R-1, Il1r1, EC:3.2.2.6, CD121a, CD121 antigen-like family member A

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Tested Applications

• Positive WB detected in: mouse liver tissue, EL-4-B5 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84094-6-RR targets IL-1R1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 1 receptor, type I
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 75-100 kDa
• GenBank Accession Number: NM_008362.2
• Gene Symbol: Il1r1
• Gene ID (NCBI): 16177
• RRID: AB_3671660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13504-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-1R1 (IL-1 receptor type 1, also known as p80, IL-1R-alpha or CD121a) is a 80 kDa transmembrane glycoprotein and a member of the interleukin-1 receptor family (PMID: 35163653; 29248002). It is a receptor for IL-1α, IL-1β, and interleukin-1 receptor antagonist (IL-1Ra). IL-1α and IL-1β interact with the extracellular domain of IL-1R1, triggering the recruitment of an accessory receptor, the IL-1RAcP, resulting in a functional receptor complex that initiates IL-1R1 signaling cascades (PMID: 35163653). IL-1Ra competes with active IL-1 and blocks binding to their common activating receptor IL-1R1 and, thus, inhibits IL-1 signaling (PMID: 35332327; 11641511). It has been reported that IL-1R1 may also bind IL-38 (PMID: 29248002). IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be released from the cell surface following proteolytic cleavage, generating a soluble form of 55-60 kDa (PMID: 17324958).