Anti-Mouse IL-17F Rabbit Recombinant Antibody, PBS Only

IL-17F Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230026G6

Cat no : 98019-1-PBS

Synonyms

Il17f, interleukin 17F, IL 17F, C87042, 230026G6

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Validation Data Gallery

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  FC experiment of C57BL/6 Th17-polarized splenocytes using 98019-1-RR (same clone as 98019-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th17-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-17F Rabbit Recombinant Antibody (98019-1-RR, Clone:230026G6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98019-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98019-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98019-1-RR against Mouse IL-17F were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 98019-1-PBS is IL-17F Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 17F
• Calculated Molecular Weight: 17KD
• GenBank Accession Number: NM_145856.2
• Gene Symbol: IL-17F
• Gene ID (NCBI): 257630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.