IL-17A Monoclonal antibody, PBS Only

IL-17A Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D5

Cat no : 66148-1-PBS

Synonyms

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, interleukin 17A

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Validation Data Gallery

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  WB analysis using 66148-1-Ig (same clone as 66148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66148-1-Ig (same clone as 66148-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66148-1-Ig (same clone as 66148-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66148-1-Ig (same clone as 66148-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66148-1-Ig (same clone as 66148-1-PBS)

  1X10^6 Jurkat cells were stained with 0.2ug IL-17A antibody (66148-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66148-1-PBS targets IL-17A in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-17A fusion protein Ag3733
• Full Name: interleukin 17A
• Calculated Molecular Weight: 155 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC067505
• Gene Symbol: IL17A
• Gene ID (NCBI): 3605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.