IL-13Ra1/CD213a1 Polyclonal antibody

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  WB analysis using 27916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27916-1-AP (IL-13RA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IL-13Ra1/CD213a1 Polyclonal Antibody for WB, ELISA

Cat No. 27916-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

IL13RA1, Cancer/testis antigen 19, CD213A1, CT19, IL 13 receptor subunit alpha 1

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Tested Applications

• Positive WB detected in: HT-29 cells, Jurkat cells, Raji cells, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27916-1-AP targets IL-13Ra1/CD213a1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25542

  Product name: Recombinant human IL-13RA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-277 aa of BC009960

  Sequence: MEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMKCSWLPGRNTSPDTNYTLYYWHRSLEKIHQCENIFREGQYFGCSFDLTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYV

• Full Name: interleukin 13 receptor, alpha 1
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC009960
• Gene Symbol: IL-13Rα1
• Gene ID (NCBI): 3597
• RRID: AB_3086010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78552
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a 65- to 70-kd glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ciara (Verified Customer) (01-30-2024)

Positive staining in N2a cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Neuro-2a mouse neuroblastoma cell line